In 1972, the Author observed that there showed a marked diuresis by the hypotonic osmotic stimulation with H2O into the anterior hypothalamus of rabbit, and at the same time a strong diuretic substance appeared in the serum of the same rabbit.
Coworker Kim observed that diuretic substance appears in the convalescent serum of the nephrotic syndrome, coworker Chung observed diuretic substance in the serum of the hydronephrosis rabbit and cowoker Min observed diuretic substance in the serum of the kidney vein ligated rabbit.
On the other hand, as the volume receptor located in the central nervous system and homeostatic mechanism is performed by delivering tension of the venous system in the thorax espicially of the right atrium to the volume receptor, author ;intended to study the influences of the circulatory disturbances of the central nervous system in producing diuretic substance of the sera of rabbits.
Mfaterials and Methods.
Korean white male rabbits weighing 2.Okg were used
Urine were collected through indwelling catheter after they were well hydrated orally by gavaging with 100 ml of 2% NaCl solution three times at one hour interval to maintain positive water balance.
Sera were obtained once daily from the rabbits of which jugular vein and carotid artery were ligated unilaterally or bilaterally.
When the urine volume through the indwelling catheter exceeded 3.0 ml per 10 min. after hydration, hydrated rabbit was given intravenously iml of above serum and observed the changes of urine volume per 10 min. for over 160 minutes.
The results were as follows.
1) When the sera, which were derived at the intervals of 24 h. for 15 days after the jugular vein was ligated unilaterally, were injected intravenously in the hydrated rabbits the urine amount showed marked increase of 8.0^9.0 ml during 10 minutes in 2 cases, there showed moderate increase of 7, 0.8.0 ml in 5 cases and no distinct changes were observed in 4 casts.
2) When the sera, which were derived every 24 h. for 10 days after th,, ligation of the jugular vein bilaterally, were injected in the hydrated rabbits, marked increase of urine amount of 8.0 x-9.0 m! during 10 minutes were in 5 cases, moderate increase of amount to 7.0^8.0 ml were in 3 cases, and in only one case there was no marked change.
3; When the sera, which were derived every 24 h. for 10 days after the ligation of the carutid artery unilaterally, were injected in the hydrated rabbits, the marked increase of urine amount of d. 0^-9. 0 ml during 10 minutes were in 2 cases, moderate increase of 7, 9 -8. t^1 ml were in 8 cases and in 4 cases there were no marked change.
4) Whea the sera, which were d-rived every 24 h. for 7 days after the ligation of the caratid
artery bilaterlly, were injected in the hydrated rabbits, marked increase of urine amount to 8.0^9.0 ml during 10 minutes .was none, moderate increase to 7.08.0m]. were in 3 cases, and no changes were observed in 3 cases.
The results observed above show that there appear strong diuretic activity in the serum of the rabbits after the jugular vein was ligated bilaterally. This leads us to the conclusion that circulatory disturbances in the central nervous system have an influence in producing the diuretic substance in the rabbit.
|